698 
of numerous small violet platelets. The experiment can be made in 
a test tube and also on a microscope slide. Under the microscope 
the process of solution, the yellow-coloration in and round the cells 
and the separation of the violet platelets can be seen. 
The phenomena observed can be explained in the following way. 
The orange-yellow carotinoid is fairly easily soluble in acetone or 
absolute alcohol; the other one is practically msoluble, but its solu- 
bility is increased by the presence of the orange-yellow one, with 
which it forms mixed crystals. A solution of both is produced in the 
cells, and is quickly diluted, and this brings about that the carotinoid 
insoluble in acetone or in absolute alcohol separates out. I am con- 
firmed in this opinion by an observation cf Zoer *). When he extracted 
the yellow carotinoid from the aqueous solution of the saponification 
products with petroleum-ether, the other separated out beneath the 
petroleum-ether. 
I cannot distinguish any definite form in the violet platelets. They 
behave in the following way towards reagents and solvents. With 
sulphuric acid of 66'/,‘/, the colour is not modified or only slightly 
so, but with 76°/, sulphuric acid the crystals quickly take a blue 
colour and this is speedily followed by dissolution. In a saturated 
zine chloride solution in 25°/, hydrochloric acid and in a saturated 
antimony trichloride solution in 25°/, hydrochloric acid they hecome 
blue, then the crystals generally deliqnesce to blue globules and 
dissolve. The solutions are bluish-violet or blue. With bromine water 
a very transitory bluish-green colour is observed. In a solution of 
phenol in glycerine (3 to 1) the crystals dissolve, whilst the solvent 
becomes bright reddish-violet. 
If the reddish-violet crystals obtained from the red aplanospores 
by means of Moriscn’s reagent are compared with those separated 
out from alcohol and acetone and with the orange-yellow ones 
obtained from the green aplanospores by Moniscu’s reagent, then the 
first mentioned crystals, so far as their properties are concerned, 
must be placed between the other two, and this strengthens my 
belief that they are mixed crystals. 
l must here remark that according to Zopr*) the violet-red or 
blood-red carotinoid enter into combination with potassium hydroxide. 
On this account it should be assumed that the reddish-violet crystals, 
separated out with Moriscn’s reagent contain the potassium compound 
of the carotinoid and that the crystals obtained with acetone and 
alcohol consist of this compound. In the microchemical investigation 
1) Le. p. A139: 
2) lic. p. 419. 
