756 
Each culture tube was filled with 50 cm’. of the above liquid 
and coppersulfate was added in different concentrations. 
After boiling spores of Aspergillus niger (Form I) *) were inoculated. 
The observed development is described. in Table III; the formation 
of but few spores is caused by the use of the said chemicals free 
from manganese, as is further explained in Table V. 
tA BT EG 
Temp. 34—35° 
& 
| Coppersulfate added 
(Cu SO4.5 Aq.) 
Course of development after 
Nr. 
ha In Gr. : 
In milligr. 5 oe ee | | 3 5 9 days 
| | 
| rather vigorous, ,; . 
gorous, hardly| vigorous, only 
ah Comics a | nd nbr ‚any spores few spores 
| | 
| 8 | oa a vigorous, very 
- 0,01 10 000 000 if | =i jk few spores 
8 | vigorous, hardly 
| SSS == == ? 
: pe 1000000 | + | 5 | any spores 
| 8 ce | | 4-44, hardly [4 hardly 
a Le 100000 | Ed Ae: | any spores any spores 
| 2,8 ++, hardly | +4, hardly 
5 | 5,5 10 000 + ae any spores any spores 
| 7,2 | S++, hardly +++ +, hardly 
6 | 9 10 000 a ata any Spores any spores 
| | | 
2,12 | 4+ hardly | ++, hardly 
7 26,5 1000 a | ites agate any spores any Spores 
| Ae PE Ht, few |+, hardly 
8 | 52 1000 cm sir spores any spores 
8 fea? A, few | +++, hardly 
9 | 100 1000 | a | zin spores any Spores 
| | 
| | 
5 2 | +, hardly any 
10 | 252 ene a ? | ae ? "spores 
| | 
1 oe | Ps ae after two months’ growth, 
I 505,5 100 ar but probably with mutation 
12 | 1000 | ao | = = = — 
| 100 | 
1) Compare H. J. WATERMAN. These Proceedings June 1912. 
