10000000 
diminishes the production of spores. 
The velocity of the mycelium formation as well as the assimilation 
of glucose are also slackened by the coppersulfate (Comp. Nr. 4 and 
the following Nrs with Nrs 1-3). 
By determining the quantity of dry substance‘) and the carbonic 
acid obtained from it by combustion on one hand, and on the other, 
by determining the polarisation?) and the reduction number by 
titration after Feuring, by which tbe assimilated glucose could be 
computed, the plastic aequivalent of the carbon could be fixed. 
In Table IV the results of these experiments are united. 
We see that already gr. mol. coppersulfate strongly 
TABLE IV). 
Metabolism of Aspergillus niger under influence of 
different coppersulfate concentrations. Nine days 
after inoculation. 
~ 
| Obtained |. haar of Assimilated | Plastic 
Nr. dry weight a: mould’ _ _ jaeq. of the 
| in milligrs. aterial ‚glucose in °/)| carbon. 
1 | 306 | 530 100 36,09/, 
2 318,5 556 100 | 38,0 ,, 
3 | 325,5 563,5 100 | 38,5 ,, 
4 377 643,5 100 44,0, \ 
a | 148 ZE 52 34 ,„ 
6 190,5 331 57 39,5 ,, 
7 83 146 31 oe - 
8 | 412 192,5 32 4l , 
9 89,5 158.5 26 415, 
10 6,5 o 6 = 
MW) — = 0 =A 
12 | = — 0 — 
1) Dried at 105° to constant weight. 
*) Determined by the saccharimeter of Scumipr and Haenscu. It is proved 
that in the Nrs. 1—6 a small quantity of a hitherto unknown polarisating sub- 
stance occurred, not reduced by ‘FEHLING”’. 
3) Compare Table III. 
49* 
