na 
Young colonies dewdrop-shaped; highly glistening, conden- 
sation water as in agar-cultures. 
Broth-culture: slow growth, cloudy with sediment, which 
squirms up like a slimy flagellum when shaken, and may be 
equally distributed. No pellicle is formed, as is the case in 
broth mixed with horse-serum, yeast-decoctum or ascites-fluid: 
LourFLER’s serumsmear-culture, growing abundantly 
in 24 hours, even, very slimy. 
Plate-culture (24 hours): highly elevated above the 
surface; colour deep canary-yellow, later in part brownish-red, 
round, smooth-rimmed, uniformly finely granular, dewdrop- 
shaped, moist-glistening, condensation water very cloudy; no 
pellicle. 
Milk is not coagulated ; ultimately a pinkish coloration. 
Glycerin-potato-culture: growing badly ; hardly visible, 
light yellow; dim-glistening. 
Blood-glycerin-potato-agar (Bordet medium): 
Smear-culture: abundant growth in 24 hours; tbe culture 
first obtained was greenish, afterwards rather brown to brown- 
ish-black, chocolatelike, elevated above the surface; very 
slimy; easy confluence of colonies; glistening; condensation 
water cloudy. 
Sporeformation not noted. 
Resistance to: 
Desiccation: cultures in fluid media did not lose vitality 
at room-temperature 11 weeks after drying. 
Heating for half an hour at 60° C. kills off the culture; 
when heated for 5 minutes at 80° C. they are also destroyed. 
Cold: cultures exposed for 4 hours to a temperature of —60° C. 
did not lose vitality. 
Light: diffuse daylight does not kill the microbe; nor does 
it affect growth. 
Lifetime: After 16 weeks the cultures have not yet died away. 
Chemical conversions. 
Formation of Gas: none in broth with glucose or lactose, 
neither in nutrose with canesugar. 
Acid-produetion: in nutrose with glycose, mannite, mal- 
tose or canesugar. 
Alkali has been detected in broth with yeast-decoct. After 
5 weeks 1 eem. °/,, n. acetic acid on 9 cc. of broth with 
yeast-decoct. appears to be just neutralised. 
H,S is not produced. 
50 
Proceedings Royal Acad. Amsterdam. Vol. XY. 
