320 



3"'. What happens tiiially to the sensitized cells; what do the 

 leucocytes do. 



Let us first answer the first question. 



Here we must ask at once if there is couiplement in the sub- 

 cutaneous lymph. 



As far as I know H. Schneider's') researches about this subject 

 are the best; he found that the tissue lymph which is obtained by 

 bringing a piece of cottonwool under the skin, and afterwards 

 pressing it out, contains very little complement indeed. One always 

 finds a little more complement than woidd really be the case if 

 we had [nire tissue lymph; a slight mixing with serum can of 

 course hardly be avoided. It goes without saying that in this 

 way we cannot be certain to get a liquid, agreeing with the tissue 

 lynq)h; the piece of cottonwool naturally works irritating; an 

 inflammation arises. But the injection of the corpuscles also causes 

 an inflammation, aud as such these two processes are equal. 



I have also made some complement titrations to the guinea pig 

 and rabbit, of subcutaneous fluids obtained in this way. 



For the solution of my haemolytic system 1 needed : 



I. Fresh guinea pig serum \/,„„ c.cm. 



Subcutaneous fluid Vso ^'-cm. 



II. Fresh guinea pig serum '/so c.cm. 



Subcutaneous fluid Vso c.cm. 



TIL Fresh rabbit serum y^ c.cm. 



Subcutaneous fluid 0,6 c.cm. no haemolysis! 



Slowing fluid 0,6 c.cm. ti'ace of ,, 



So we can affirm Schneider's experiments and assume very little 

 or no complement to exist in the subcutaneous cellular tissue ; and 

 we need not be astonished about the sensitized corpuscles not dis- 

 solving, when being injected subcutaneously. 



Now we must answer the second question. The sensitized cells 

 remaining iu the same place was supposed to be due to the agglu- 

 tination which always accompanies the sensitizing. I did not succeed 

 in obtaining an immune serum prepared in the usual way, which 

 did not at the same time agglutinate. As 1 did not know any 

 method to separate amboceptor and agglutinin when I started my 

 experiments, I took another way to prove that the remaining of the 

 hloodcells was owing to their being agghilinated and not to the 

 sensitizing. I therefore agglutinated the bloodcells in a different way, 

 and now found that clinically and histologically the same was to be 

 seen after injecting these corpuscles as after injecting sensitized (and 



1) Arch. f. Hygiene 70. p. 40 seq. 



