322 



left under the eonjniictiva of rabbit 172. Tlie serum of this rabbit 

 contains neither amboceptor nor agglutinin in a noticeable quantity 

 against sheep-erythrocytes. 



After one day there is a distinct ditFerence. There is very little 

 swelling and redness (+) on the left, but very strong swelling and 

 bluish-red change of colour (+ ++) on the right. The next day the 

 ditference is a little less, but still it is distinct. 



Conse{]uentlj it was desireable to obtain a serum which sensitized 

 strongly (at least '/,„„J. but which agglutinated little or not at all. 

 As there was no question of a chemical separation — all the litera- 

 ture tells us that all suchlike attempts lead to no result whatever — 

 Such serum had to be obtained in a different way. In the literature 

 about the helerogenetical antibodies is mentioned that serum of a 

 rabbit which had been in some way prepared in order to get hetero- 

 genetical amboceptors against sheep-erythrocytes, would then contain 

 no more agglutinins than ai-e found in normal rabbitserum. 



My experiments in this direction have however not yet led to the 

 desired result. One rabbit which was injected with cattle-erythrocytes 

 had a serum with litre V200 against cattle-corpuscles, and a litre Viooo 

 against sheep-corpuscles. However it very clearly contained agglutinins 

 against the latter. The same thing appeared with two rabbits which 

 had been injected with horse-kidney extract. The litre against sheep- 

 blood was 7,0, of both of them. Both distinctly contained agglutinins, 

 if only little. The sheep-corpuscles treated with this serum i-emained 

 for some days in the same place, after having been injected under 

 the conjunctiva. 



So in this way I could not prove with certainty that the agglu- 

 tinin is the cause of the prepared corpuscles remaining under the 

 conjunctiva. ^) 



III. I will now mention some experiments which have been made 

 in connection herewith, but which do not directly bear upon the 

 subject mentioned in the title. I have asked myself whether the same 

 ditference as is mentioned above, is also found when non-prepared 

 bloodcells are injected subconjunctively partly with prepared, partly 

 with non-piepared animals, and whether here too the agglutinin was 

 of any importance as to the remaining of the erythrocytes. And this 

 has indeed appeared to be the case. 



Rabbits with serum containing amboceptor (and agglutinin) still 

 show a strong swelling under the conjunctiva after one or two 

 days after having been injected with the erythrocytes in question 



1) Note added during to the correction : Now 1 had more success with this 

 experiment. The helerogenetical serum which 1 now used was Viuoo strong. 



