(in in}' experimenls they wore liorsecorpnscles), wliereas the oontrollirig 

 animals showed hardly any swelling after one, and no swelling' at 

 all aftei' t\vo days. In accordance with this the tissne tlnid (obtained 

 in the above mentioned way wiih cottonwool) obtains amboceptor 

 as well as agglutinin, if thev arc in the serum. 

 Rabbit 160 immunized against, cavia-erythnx'ytes. 



Serum agglnlinalion Vsn and)oceptor 7io weak (-]-+) 

 fluid „ \,',o „ Vio - (+)■ 



Rabbit 192 immnnized against horse-erythrocytes. 

 Serum agglutination ',„ amboceptor '/<,„ 

 fluid ,, Viu weak ,, Vzo nearly 



Rabbit 147 immnnized against cattle-erythrocytes. 

 Serum agglutination Vs amboceptor ^/^^ 

 fluid „ Vs ,, 'Ao- 



I have now investigated if it really is the agglutinin which deter- 

 mines the difference. 



Rabbit 116 agglutination strong, amboceptor V.oo- 



Rabbit 148 „ very weak, „ \f,,. 



Both rabbits are subconjunctively injected with \'^ c.cin. washed 

 sheep-erythrooytes. 



After one day there is a very strong bluishred swelling with 116, 

 with 148 hardly any swelling; after 2 days still a strong swelling 

 with 116, with 148 nearly all the blood has disappeared. 



A stronger proof is given by the rabbits that were injected with 

 horsekidney extract ^). Although the tilre against sheepcorpuseles was 

 not high here (with both Vïoo) ^^ gi"eat difference was stated with 

 the controlling animal (titre also \^2on)- 



After one day hardly any blood was to be seen with the first, 

 contrary to the controlling-animal. 1 think these experiments are 

 of some importance. For in the latest great report about the 

 agglutintition known to me, that by Paltauf^), the author says on 

 p. 515: Ob Agglutination audi im Oi'ganismus statlfindet erscheint 

 recht zweifelhaft. 



At least I l)elieve 1 have proved the haemagglutination to take 

 place in the subcutaneous tissue. 1 oidy want to insert here that 



1) These are the .same animals as were mentioned above: their serum did 

 contain agglutinin, but much less than tlie animals immunized in the ordinary 

 way. That here we got no agghitinin el'i'ect, and that we did when mixing the 

 serum with the bloodcells in vitro, may be explained by the fact that the agglu- 

 tinin can pierce with so much more dillictilty into the tissue fissures and reach the 

 bloodcells than when a great quantity of serum in vitro is directly added. 



2} KoLLE und Wassermann, lie Auflage, II, p. 463 — 054. 



