\V2-i 



the phenomena mentioned above belong to the department of local 

 anaphylaxis (Pijenuinenon of Artihs). As fai- as I ivnow they have 

 not been studied as to the immunisation with bloodcells ; they have 

 with serum or bacteria. Tiiis realiy is only a question of name 

 however: the essence of local aiuiphylaxis is still as unknown to 

 us as that of general anaphylaxis. 



In any case we can see by the bloodcells that the disintegration 

 of albumen is a vevy slow one; I do not wish to deny however, 

 that part of the flood of leucocytes is owing to this disintegration. 

 What has been stated somewhere else viz., a primary necrosis of 

 the tissue and after that an infiltration of leucocytes '), 1 have never 

 obser\'ed ; I could sometimes also state a toxical influence of the 

 injection out of an oedema of the cornea: but this happened very 

 rarely. Then one should not directly compare the phenomena of 

 subcutaneous injection with those of intracorneal injection (Wessely, 

 VON SziLY); in the latter case the current of fluid is much slower, 

 so that great differences can occur by this. It would however lead 

 us too far if we entered into this more closely. 



We must now still treat of the third question : what happens to 

 the sensitized (agglutinated) cells, and what do the leucocytes do in 

 this process? I must first of all mention that 1 could not find any 

 difference between histological images when injecting sensitized or 

 only agglutinated bloodcells. This, however, is in keeping with 

 other experiments. For, there being a great difference in vitro between 

 the phagocytosis of sensitized (opsonized) and nonsensitized cells, — 

 the former ai-e phagocytated, the latter are not, when brought 

 together with suitable leucocytes — one does not find back this 

 difference in vivo when injecting the cells into the abdomen, 

 previously injected with broth. Achakd and Foix *) some time 

 ago tried to find the causes of this difference, but in vain. I did 

 not succeed either '). We need not be astonished however, when 

 finding the same conduct in the subcutaneous tissue as in the 

 prepared abdomen. 



Are the erythrocytes now phagocytated ? Notwithstanding my 

 observing a great many preparations, I did not succeed in getting any 

 certainty whatever about this in my histological sections; to form 



1) H. FucHs und Meller, Z. f. Ophlhalmologie. Bd. 87, p. 280. 



2) AcHARD and Foix Arch, de Médecine expérimentale et d'anatomie Patholo- 

 gique, January 19i4. 



3) Prof DE Vries advised me to add to the mixture (foreign bloodcells, fresh 

 serum (witliout opsonins) and leucocytes) scrapings of the peritoneum endothelium; 

 with this I had no success either. 



