328 



Rabbit after IV, hour after 1 day after 3 days after 5 days 

 contains \ i contains \ / contains \ / contains 



horse serum j [ horse serum I i horse serum j i horse serum 



+ + + - ++ - + + 



+ + - ++ }- + + 



+ + + - +^ ]- + + 



after 7 days. aft. 10 ds. aft. 12 ds. aft. 14 ds. aft. 17 ds. 



contains . 

 1 horse serum i 1 .S S 1 



155 —\ + /V.ü\|i/ 'A«ü '/looo w. Vi„„ 



156 — ] ++ \ Vio f 1 i Vioo weak Vi„o w. Vioo 



157 -J - i Viol «I i V:oo ? V.o W. 



158 

 159 



158 -f ++ ) Vio f I g 1 Vioo Vioo Vioo 



Here too we also see some irregularity: (rabbit 157 immunizes 

 somewhat less than the other, but even a mixture of 25 times more 

 antiserum than serum slill has immunizing effect. 



I did not try if surplus of serum can do any harm when 

 immunizing, for one tlien gets too great quantities so that it is hard 

 to inject them: 50 ccm. serum is rather much for a rabbit. 



These experiments seem to be somewhat contrary to a communi- 

 cation of Dorr (report about Anaphylaxis, Kolle und Wasserm. 

 He Aull.), that the precipitate obtained by mixing serum and anti- 

 serum, has no immunizing effect. But this is only a seeming 

 contradiction. For, according to investigations e.g. by Welsh and 

 Chapmann') this precipitate only contains traces of parts of the 

 serum and it is almost exclusively formed out of the antiserum. 



Thus 1 have found that of a serum of a rabbit which was 

 immunized against human serum (titre Viooo) 75 ccm. was necessary 

 to form together with 1 ccm. human serum (together till 150 ccm.) 

 a precipitate, so that in the above mentioned liquid no more human 

 serum could be indicated with my antiserum (Viooo)- ^ ^^m- being a 

 very small dosis to immunize a rabbit, it is clear that not much 

 can be expected in general from an injection of the precipitate'). 

 I have now also examined the local effect of serum and antiserum. 



1) Zeitsch. f. hnmunitalsf. 9, p. 517. 



2) I here give up the question whether there is any human serum at all to be 

 found in the precipitate, or whether it could be again removed by washing. 



