140 Mosquitoes 



Punctipainis may deposit more than 2000 eggs 

 during a single breeding season, with but a single 

 fertilisation, when supplied with blood. 



From ether experiments it seems that A. pnncti- 

 pennis at midwinter in Louisiana will lay eggs in- 

 doors in 3 to 7 days after feeding, that the eggs will 

 hatch in the laboratory in 8 to 10 days, and that 6 

 or 7 batches are most frequent, but as many as 9 

 have been deposited. One fed January 26 laid be- 

 tween this time and February 20, 6 batches of eggs, 

 the first ovulation occurring 4 days after the first re- 

 past, and the others from 3 to 5 days after each suc- 

 cessive feed, one meal to a batch. She fed the same 

 day she laid, immediately after oviposition if she had 

 a chance, and escaped after her seventh meal. An- 

 other laid 8 batches at intervals of 4 to 7 days, feed- 

 ing about the same way except that, having de- 

 posited eggs and been fed February 24, she laid 

 her fifth and sixth batches February 28 and March 6 

 without a meal between them. Her last batch was 

 February 10, and she fed immediately after. 



The winter period of incubation was found to be 

 from 2 to 58 days, generally (in the laboratory) 10 

 to 18. The winter pupae came 18 to 25 days after 

 hatching, the pupa stage lasting from 2 to 4 days. 

 The time between a meal and laying is 3 to 6 days ; 

 punctipainis generally takes 4 days, sometimes 5. 

 One of this species, captured December 12, not fed 

 but put in a bottle with water, was active on the 

 19th, laid on the 20th, and died on the 21st. The 

 eggs hatched February 6 to 8. In summer the cycle 

 may be completed in 16 days, possibly less. 



