200 Mosquitoes 



shallow dishes with Spirogyra and protozoa to eat. 

 I always keep a big jar with Euglena and other pro- 

 tozoa in it all the time, as a base of supplies. The 

 larvae should not be set in strong sunlight. They 

 may easily be handled by the pipette, the mouth of 

 which should never have sharp edges. The move- 

 ments of the bottom feeders may best be observed 

 through the sides of the jar ; an ordinary thin tumbler 

 may advantageously be used for this purpose. For 

 the other forms, shallow dishes are better. When 

 the pupae form, they may be removed by the pipette 

 to wide-mouth bottles ; old vaseline bottles are good, 

 with cheese-cloth over the top and about an inch of 

 water in the bottom, no weeds. 



Eggs of Citlex are easily obtained by setting pails 

 in the yard. The rafts may be put in the breeding- 

 jar and the development observed. 



Careful records are an absolute necessity. In tak- 

 ing adults, record place and time of capture, date, wind 

 if many captures are being made at the time, tem- 

 perature, and weather ; also, if there is a swarm, and 

 if mating is going on. If the mosquito is fed, record 

 that ; date, time of day at which it feeds, how many 

 meals it makes, time of laying eggs, time of hatch- 

 ing, kind and number of eggs, whether they need 

 agitating to hatch, whether they all hatch at once, 

 how many batches are laid, length of larval period, 

 any sudden fluctuation of temperature which may 

 affect this, food and habits of larvae, length of pupal 

 period, length of life of adults, and habits as far as 

 possible. 



The best preservative for larvae, pupae, and eggs is 



