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parative proteolytic power, after the method of Grirzner*) by mixing 
1 ee. of the fluid with 9 ce. 0,1°/, HCI to which, at least 10 minutes 
before, 50 mers of finely divided earmin fibrin had been added, then 
turning the tube once every minute, filtering the contents after a 
certain time through glasswool and establishing the intensity of its 
colour with the aid of Grirzner’s colorimeter against a solution of 
carmin fibrin in pepsin hydrochloric acid. 
First of all the proceeding of the enzyme was compared in agar- 
agar with and without fibrin. With a view to this in each of two 
tubes, containing agar-agar, 10 CC. was added of a mixture of equal 
portions of 3°/, agar and 0,2°/, HCl, and in two others 10 CC. 
of a mixture of 3°/, agar and 0,2°/, HCI with carmin-fibrin that 
had been rubbed down very tinely and had swollen in this acid. 
After three days one of each couple of tubes was opened and 
examined in the manner described. Just as in all the following 
experiments I designates the lowermost cylinder, the one nearest 
to the pepsin-agar; II the one next to it and III the topmost cylinder. 
The result was to this effect: . . 
| Division Division aa: 
Weight | Milk | mark |Weight| Milk | mark |Weight| Milk | Division 
mn F 3 : = k 
(grms) | clots in| Colori- | (grms) | clots in | Colori- | (grms) |clots in gar) 
NE meter | Colorimeter 
with fibrin | 1 2.04 | 2 min. | 1.2 I] 2.14 (15 min.| 0.5 | II 2.0 (30 min. Pa, AO 
without » hes} 4d ~~ Ie? Reiss no 0 Er 0 
clotting clotting 
After 6 days the other two tubes were opened. Now we found: 
p : 5 = 5 t 
with fibrin | I 2.48 |3!/, min.) 1.1 |I 2.54 |10 min.| 0.8 | III 8.24/90 min. AOE cable 
without » 12.044 » 1.0 ‚|I 2.04 | no 0 III 2.0 no 0 
clotting clotting 
The 2nd experiment was conducted in the same way. Result after 4 days: 
with fibrin | 11.80 (2 min. | 4.1 111.95 {17 min.| 1.1 {III 2.00/40 min. 0.5 
; not 
without » 12.10 2, » 3.1 I] 2.00 [18 » 0.5 | III 2.00 etdttine measurable 
After 13 days: 
with fibrin | I 1.20 (13/4, min.| 2.8 111.54 | 4 min.) 2.4 | III 1.60/17 min. 2 
without » [15060124 > 2.6 111.94 14 » Peas IE 19060 ‘> 0.1 
1) Vide GeseLscHaP, Zeitschr. f. Physiol. Ghem. XCIV, 205 and Onderz. Physiol. 
Laborat. Utrecht, 5e R. XVI, 198. 
