274 
I have desisted from a determination of the absolute pepsin amount 
of the several columns, though this could be done by comparison 
with a solution of pepsin of known strength, since the pepsin in 
each column was not divided evenly, but lessened considerably 
from the bottom upwards. The values given show distinctly enough, 
that the enzyme rose in the agar without protein as well as in the 
agar with protein, but in the latter more considerably. Only after 
13 days could it be demonstrated that the enzyme had reached the 
upper column in the protein-free gel. 
As regards the absolute value of the figures it will not do to com- 
pare the results obtained on various days, because the milk used 
was different every time and for comparison in the colorimeter 
every time another solution of carmin-fibrin was taken. 
Similar results were achieved with clotted white of a hen’s egg: 
White of a hen’s egg, diluted with 10 times its volume of water, 
was beaten up and coagulated by boiling under addition of acetic 
acid to a very weak acid reaction. The flaky precipitate was filtered 
off and washed with water. Part of this was put in 0,2°/, HCI 
and evenly distributed in the fluid by rapid shaking. In each of 
two tubes with pepsin-agar was added 5 CC. of this protein- 
containing acid, mixed with 5 CC. 3°/, agar in two other tubes 
vC °/, lagar swith BCC 0,2 0/5 HCI. 
Two tubes examined after 3 days: 
Colori- 
meter Weight 
Clotting Colori- Weight 
Weight ctotng meter 
Cotting weit 
* 
with protein I 2.64 |13/, min.| 2.2 [Il 2.56 | 22 min) 0.4 {III 2.35] 40 min. vel Te 
not meas- | 111 2.40 
urable Os 0 
without » | 12.70|2 » | 2.0 |II 2.56 |130 „ 
The second set of two tubes got lost. 
In every tube so much hydrochloric acid had been put, that the 
content of the gel was 0,1 °/, over the whole tube. Here, however, 
we had to consider that in the tubes containing the protein, the acid. 
was partly bound, so that the concentration of the H-ions in the 
«agar-protein gel was undoubtedly lower than in the agar gel without 
protein. The observed differences could, however, hardly be attributed 
to it. If the movement of the enzyme depended exclusively upon 
diffusion, it might presumably be promoted by an acid reaction, 
considering that, during the sojourn of the tubes in an environment 
of 27° C., the acid attacks and softens the agar. In every experiment 
therefore, the protein-containing agar was more solid than the protein- 
