277 
After 3 days: 
1.0 
Weight Clotting coor Weight | Clotting Color) Weight Clotting 
with album.| I 2.35 |l min.) 3.3 II 2.40| 7 min.| 2.1 III] 2.40) 22 min. 
without » | 1 2.20 |1!/4 » 3.2 If 2.2035 1.0 [III 2.15} none 0 
After 4 days: 
with album.) I 2.05 | 50 sec.} 3.5 ll 1.95. |} 2 mms | S22 III 1.98) 4 min. 2.5 
without » 1 2.04 | 1 min.| 3.1 1 2.00|6 » [2 III 2.08; none 0 
Deutero-albumoses, 100 mgrs. 
After 2 days: 
with album.| I 2.40 |1'/, min.) 5.5 I] 2.44 |15 min. | 2.5 Il] 2.44] 1 hour 1.0 
without » 1 2.40 |11/, » 4.5 II 2.34} 1 hour} 1.0 III 2.30) 2 hrs 0.6 
After 3 days: 
with album.| I 2.24 |1 min.| 3.0 I] 2.10 |2'/, hour] 0.5 Ill 2.14) none 0 
without » | I 2.20 [I!/4, » 3.0 I] 2.34} none | 0.2 /|III 2.20} none 0 
The solution contained in 11 CC 0.2°/, HCl, 75 mgrs. tryptophan, 
7.5 mgrs. of cystin, 40 mgrs. of histidin, 70 mgrs. of tyrosin and 
30 mgrs. of alanin. On heating to 40° C. the solution was almost 
clear. Of this solution See. and See. of 3°/, agar was put in each 
of 2 tubes. In the other 2 tubes 5 cc. of 0.2°/, HCl was put, 
together with 5 ce. of agar. 
In the tube heated for 4 days at 27° C. the gel without amino- 
acids, which, therefore, had been more exposed to the action of the 
acid, was very soft. Perhaps it is owing to this that the pepsin has 
penetrated farther than is generally the case in the agar without 
protein. 
It might be surmised, that from the experiments described it does 
not even follow that pepsin is indeed competent to diffuse in pure 
agar, seeing that a gel of this agar prepared in the usual way, will 
always contain nitrogenous substances, which may belong to the 
group of proteins. I believe this is an unjustifiable assumption. It 
is difficult to ascertain whether the agar-gel or sol contains protein’ 
because sensitive reactions on protein cannot be successfully applied 
Colorim. 
