44 MONICA TAYLOR 



extremely sensitive to change of position, temperature, or light. 

 When, therefore, a spherical amoeba is removed from its culture 

 for purpose of examination it quickly accommodates itself to 

 its new surroundings, and crawls away without completing the 

 division of its nucleus. Very complicated appearances are 

 due to such nuclei rolling about in an active amoeba. With 

 care to preserve their cultural conditions, however, spherical 

 amoebae can be removed and isolated, when they are seen 

 to give rise to daughter products. 



These ' lobed ' nuclei, however, are formed before the 

 amoebae become spherical. They are very early stages in the 

 division process, for although the actual separation into 

 daughter nuclei takes place when the amoebae are spherical, 

 the division is initiated at a much earlier stage. Most of 

 the large individuals in a watch-glass culture will be foimd to 

 have a ' lobed ' nucleus. If they become spherical this is 

 converted into a varying number of daughter nuclei, unless, 

 as described above, external conditions disturb the process. 



Bibliography. 



1. Carter, L. A. — "Some Observations on Amoeba proteus", ' Proc. 



Royal Phys. Soc. Edin.', vol. xx, jmrt 4, 1919. 



2. " Case of Mitotic Division in Amoeba proteus ", ibid., vol. xix, 



no. 4, 1913. 



3. Dofiein, ¥. — ^" Die vegetative Fortpflanzung von Amoeba proteus, Pall.", 



' Zool. Anzeiger ', Bd. xlix, no. 10, 1918. 



4. Schaeffer, A. A. — " Note on Specific and other Characters of Amoeba 



proteus, Pall. (Leidy), A. discoides, spec, nov., A. dubia, spec, nov.", 

 ' Arch, fill Protistenkunde ', Bd. xxxvii, 191 6. 



5. Taylor, M., and Hayes, C. — " The Technique of Culturing Amoeba 



proteus ", ' Journal of the Royal Microscopical Society ', pp. 241-4, 

 1921. 



