78 w. n. f. woodland 



Notes on Technique. 



I fixed spocimens of Amphilina paragonopora oither 

 in Zenker's fluid, Mann's fluid, aceto-bichromate, or simply 

 in G per cent, formalin. Some of the specimens were first 

 flattened between glass slides, and these, when properly 

 stained, showed the reproductive organs well. Unflattened 

 specimens were either embedded for section-cutting (the 

 specimens being kept straight by being placed between small 

 squares of wire gauze while in the embedding bath) or simply 

 preserved in formalin and glycerine. Whole specimens and 

 sections were usually stained either with Delafield's haema- 

 toxylin (diluted with ten times its bulk of water, and immer- 

 sion over-night) and in some cases followed by eosin or other 

 plasma stain, or with borax carmine. Both stains gave good 

 results. 



In conclusion I wish to express my indebtedness to Dr. H. A. 

 Baylis, who very kindly consulted for me, while I was in India, 

 several original papers and checked my references to previous 

 work, and to Dr. S. W. Kemp, who kindly sent to me some 

 volumes from the Indian Museum library at Calcutta while 

 I was in Allahabad. I am also indebted to Colonel G. E. F. 

 Stammers for some assistance in checking references, and to 

 Messrs. B. K. Das, M.Sc, and S. K. Datta, M.Sc, for the 

 considerable assistance they have kindly given to me in o})tain- 

 ing large numbers of Macrones and finding Amphilina. 



Summary of Principal Conclusions in Parts I and II. 



1 . A m p h i 1 i n a paragonopora is parasitic in the body- 

 cavity of the Siluroid fishes, Macrones aor and M. seen- 

 ghala, from the Ganges and Jumna, India. It is linear in 

 shape and varies in length from about 10 mm. to 280 mm., 

 but full-grown larvae are not formed in the worm until it is 

 at least 100 mm. in length. The ' anterior ' end of the parasite 

 is rounded and carries a small proboscis, which is a boring 

 organ and not a sucker. The uterus opens anteriorly to the 

 left and at the l)ase of the pro))oscis. The ' posterior ' end of 

 the bodv is marked l)v a semicircular inlet or liav, and on 



