THE INTESTINAL PROTOZOA OF FROGS AND TOADS. 227 
(58), and furnishes some interesting details. Before division 
the axostyle (“ mestolo”’) is absorbed. Then a _ spindle 
(“‘fuso”’) of unknown origin makes its appearance beside the 
nucleus. It elongates enormously and comes to lie between 
the daughter nuclei; and subsequently a portion of it at least 
takes part in the formation of the axostyle in the two 
daughter individuals. It seems quite probable that a con- 
dition identical with that seen in the trichomonads really 
exists here, but that it was not fully made out owing to the 
great complexity of structure in Joenia. At all events, the 
comparison is very suggestive. 
Another interesting comparison may be made between the 
axostyle and the axopodial rays of the Heliozoa. Camp- 
tonema furnishes an excellent example of the connection 
between axopodium and nucleus (Schaudinn [77]) and well 
illustrates a condition analogous to that of nucleus and 
skeleton in trichomonads. 
1 
Conjugation.—From what has already been written 
regarding the life-cycle of the trichomonads from frogs, it 
will be apparent that I am quite unable to bring forward any 
evidence regarding their sexuality. At no time have I ever 
found the slightest indication of the existence of any form of 
conjugation. 
It was stated by Schaudinn (43) that a conjugation 
(heterogamic) takes place in the Trichomonas intestinalis 
inman. ‘This has never been properly confirmed. Shortly 
after, Prowazek (73) described an autogamyin T'richomastix 
and a heterogamy in Trichomonas intestinalis from the 
rat. Peculiar structures, said to be stages in conjugation 
(autogamy) in T. intestinalis in man, have since been 
described by Ucke (84) and Bohne and Prowazek (51). And 
it is to these very questionable bodies that I presume 
Prowazek refers (74) as autogamic stages. Personally I can- 
not agree with this interpretation of the structures. I have 
good reason for regarding them in a very different light. 
