254 C. CLIFFORD DOBELL. 
what they saw. I admire greatly the work of both. I merely 
draw attention to the resemblance. 
I have even found stages which might at first sight be 
thought to show a condition in which the nucleus was being 
completely analysed into chromidia (fig. 62). From compari- 
son of the “chromidia” with the micro-organisms in the 
same preparation, and also from what I have seen in the 
living animal, I have no hesitation in saying that the 
“chromidia” are really bacteria, and we are here dealing 
with a case of bacterial invasion, in which the nucleus is 
attacked as well as the cytoplasm. ‘lhe animals appear par- 
ticularly liable to the attacks of bacteria just before forming 
the cyst membrane. 
From what I have already said it will be apparent that I 
can confirm neither the statements of Brass as regards spore- 
formation, nor those of Hartmann’ regarding autogamy in 
HK. ranarum. On the contrary, I have found that the 
nucleus undergoes a_ perfectly straightforward series of 
changes leading up to the formation of a quadrinucleate 
cyst, which probably serves for the dissemination of the 
organism, . 
The nuclear divisions in Entamoeba ranarum present 
some interesting features. Division does not seem to corres- 
pond with any of the forms hitherto described. Mitosis was 
first described in an Amceba by Schaudinn (78), and he also 
gave (76) the first accurate description of amitosis in the 
genus, Similar observations have been made on other forms 
by other observers since. The nuclear division I have just 
' After writing the foregoing remarks I received Hartmann’s paper 
on an ameha (Entameba tetragena Viereck = E. africana 
Hartmann) found in certain cases of dysentery in man. Hartmann 
believes that an autogamy occurs, but from his figures I have little 
doubt that future observations on the living animal will show that 
a condition almost identical with that seen in E. ranarum—as already 
described in preceding pages—really prevails. Hartmann’s figures 
bear an extraordinary resemblance to isolated stages in the develop- 
ment of E.ranarum. (See Hartmann, Beih. 5, ‘ Arch. Schiffs. Tropen- 
hygiene,’ xii, 1908. 
