THE INTESTINAL PROTOZOA OF FROGS AND TOADS. 259 
very irregular externally and always heavily encrusted with 
foreign bodies (cf. fig. 86). Their colour is brown or 
brownish-yellow. They are not unlike those of Copromonas, 
though usually to be distinguished by their excrescences. 
As arule only one nucleus can be seen in each cyst. Once, 
however, I found a cyst containing two individuals, each with 
a nucleus (fig. 85). This is rare. 
Schaudinn found that, in order to develop, the cysts had to 
pass through the alimentary canal of the “host” animal. 
But this is not the case with the Chlamydophrys from 
frogs. Perhaps the cysts I observed were only temporary, 
and not the same as the durable structures which arise after 
conjugation. At all events, I found that moistening the dried 
feeces sufficed to cause a number of animals to emerge from 
their cysts. It appears to be immaterial whether the feeces 
are moistened with salt-solution, water, or juice from the 
intestine. In each case the cyst-wall dissolved, and the 
animal emerged and began life once more as an amoeba. (See 
Pl. 5, figs. 87-90, which show emergence of an amceba from 
a cyst.) A considerable percentage of cysts never dissolved. 
A good many showed protoplasmic streaming after the addi- 
tion of liquid to the feces, but after a time it ceased and the 
cysts showed no further signs of life. 
At the time when I encountered Chlamydophrys most 
abundantly I was unfortunately so busily engaged in working 
at other forms that I was unable to take proper care of the 
cultures. The result is that what few further observations I 
was able to make, though interesting and curious, were too 
uncertain to carry much weight. In consequence I cannot 
add anything more to the account of the life-history already 
given by Schaudinn. 
I may remark that the ‘“ Amceba sp.” which Wenyon 
describes in the mouse, in addition to Entameba muris, is 
perhaps also Chlamydophrys, or an allied form. 
