SOME OBSERVATIONS ON ACINETARIA. BF 
body, but in addition to this there are always scattered in 
the cytoplasm a number of granules of chromatin, the 
“ Tinctin-kérper.”’ 
There seem to me to be three lines of evidence showing 
that the ordinary ‘ Tinctin-kérper ”’ cannot be regarded as 
integral parts of a fragmented macronucleus— 
(1) From their behaviour during reproduction and con- 
jugation. 
(2) From their behaviour during starvation and feeding. 
(3) From their absence in parasitic forms such as Tachy- 
blaston ephelotensis to be described later. 
(1) There is no trace of a connecting thread between the 
“ Tinctin-kérper”’ such as is present in the macronuclei of 
Stentor and Holophrya, and there is no reunion to a single 
mass previous to division, such as has been described for 
some Hypotrichous Ciliates with a fragmented macronucleus. 
In dividing Tokophrya the macronucleus becomes twisted 
upon itself in the region of the future bud, but in all the 
stages of division isolated “ 'Tinctin-kérper ”’ are to be found 
both in the cytoplasm of the mother and of the bud 
feb Vill, fie.:2y. 
In conjugating Acineta papillifera at stages at which 
the macronucleus was still intact individuals were found in 
which the ‘‘ Tinctin-k6rper” seemed to be collected in the 
lower part of the theca, quite apart from the macronucleus. 
(2) Their Behaviour during Starvation and 
Feeding.—During starvation there is a tendency for the 
“'Tinctin-kérper ” to disappear, though even in the most 
complete cases (Pl. VIII, figs. 3 and 4) some remains were pre- 
sent. In Pl. VIII, fig. 4, which represented the last stage of 
starvation, the individual measured 25, in length and 18 
in breadth, a normal well-fed Tokophrya from the parent 
culture measuring 143 u by 58u. The tentacles and most of 
the cytoplasm had disappeared, but the nucleus seemed quite 
healthy staining rather more readily than in the well-fed 
forms. 
In preparations made a quarter and half an hour after 
