SOME OBSERVATIONS ON ACINETARIA. 379 
of the Suctoria :—“ Im Kapitel iiber die freie Knospung (s. p. 
1894) wurde schon betont, dass gewisse angebliche Knospen 
einiger Arten modglicherweise kleine parasitische oder com- 
mensalistische Suctorien sind, welche auf grosseren leben. 
Ebenso fanden wir es nahezu, wenn nicht ganz gewiss, dass 
endosphaerenartige Suctorien in grosseren Arten ihres eigenen 
Stammes schmarotzen.” 
Mernops.— My material was fixed either with weak 
Flemming or corrosive acetic. Of these two methods the 
Flemming seemed to give far the most faithful cytoplasmic 
fixation. The Flemming preparations, after washing in 
water, were treated with a dilute solution of H,O, in 70 per 
cent. alcohol, and then stained either in alum carmine or 
Mayer’s hemalum. 
Il. Tat Buppine or HPHELOTA GEMMIPARA. 
Before proceeding to give a detailed account of the some- 
what complicated life-history of Tiachyblaston ephelo- 
tensis, it will be necessary first to refer to the structure of 
the macronucleus and the mode of reproduction of its host 
Ephelota gemmipara, as it will only then become apparent 
how it is possible to distinguish the various stages in the 
life-history of the host from that of the parasite. 
The macronucleus of Hphelota is generally described as a 
horse-shoe shaped structure lying in the horizontal plane of 
the animal, and giving rise to a varying number of branches, 
especially towards the apical surface, on which the buds are 
formed. The reproduction of Hphelota (Podophrya) 
gemmipara has been described by Richard Hertwig, whose 
results have been confirmed in all essentials by the later 
workers upon this form. The buds first make their appear- 
ance as small swellings round the apical pole, varying in 
number according to the size of the budding individual from 
1 to 12. These small swellings slowly enlarge until they 
become elliptical bodies flattened along one surface, the 
