SOME OBSERVATIONS ON ACINETARIA. 383 
I could never find any trace of blackening after treatinent 
with osmic acid in preparations in which the host showed 
considerable blackening this view had to be abandoned ; on 
the other hand, in sections stained with heematoxylic eosin 
the granules seem to show considerable affinity for the eosin 
stain. I am inclined to regard the granules as reserve food 
stuff, as their number seemed to diminish in starved forms. 
Text-Fic. 11. Trext-Fic. 12. 
Text-FiguRE 11.—Karly stage of infection of Ephelota by 
Tachvblaston, from the living animal. (Zeiss 16 mm. apochr., 
comp. oc. 8.) 
Trext-FIGURE 12.—Stage three hours later than that shown in 
text-fig. 11. The tentacles of the host are not shown. (Zeiss 
16 mm. apochr., comp. oe. 8.) 
About three hours later (text-fig. 12) the parasite had under- 
gone one equal division and showed traces of the formation 
of a secondary bud. It seemed, as a result of examining a 
large number of preparations, that the first division is always 
approximately equal, but that this is followed by the forma- 
tion of a number of true buds which do not obtain their full 
size at once. By the next morning seven internal parasites, 
some of which were developing cilia, could be counted in the 
above-mentioned specimen, and the cytoplasm of the host 
had become reduced to a thin shell surrounding the central 
mass of the parasites. As the budding of the parasite pro- 
ceeds (Pl. VIII, fig. 6), frequently two or three chains of buds 
VOL. 53, PART 2,—NEW SERIES. 27 
