STUDIES ON CEYLON H#MA'TOZOA. 671 
line, quite a different form of multiplication can be observed. 
The first time I observed this, a slide with blood very 
strongly infected with Hemogregarines, and only relatively 
slightly with Trypanosomes, had been left overnight. Various 
alterations in the appearance of the Trypanosomes, to be 
described presently, had been noted before leaving the slide 
in the evening. Harly next morning the slide was found to. 
contain quite a number of small flagellates, just about the 
size of the Hemogregarines and very much smaller than the 
Trypanosomes. A few unaltered Trypanosomes were pre- 
sent, but no intermediate forms. The appearance of the 
flagellates strongly suggested a connection with the Heemo- 
eregarines. They had comparatively short flagella, the mem- 
brane reaching to about between one third and two thirds of 
the way up the protoplasmic body in different specimens. 
In fact, they showed a tantalising resemblance to the figure 
of the Trypanosome phase in the blood of the little owl, as 
described in Schaudinn’s well known memoir, and attributed 
by him to the life-history of Proteosoma noctue. 
The experiment was repeated several times, and the follow- 
ing development was made out, showing clearly that the 
organism arose from the Trypanosome. 
Some time after making the preparation the Trypanosomes 
begin to show various modifications in the external appear- 
ance. The length of time which elapses before the creature 
begins to yield to the altered conditions is remarkably 
variable, the time co-efficient throughout the whole process 
is in fact very inconstant. Generally speaking the organisms 
remain unaltered for about an hour and a half. The altera- 
tions in appearance culminate by the complete loss of the 
Trypanosome shape and the rounding off of the organism, but 
this condition is arrived at in various ways. Some of the 
Trypanosomes simply become much thickened at the non- 
flagellate end. Many become bent upon themselves, and the 
two limbs of the bend then fuse together (text-fig. 1,c and D). 
The text-figures illustrate these appearances. The myo- 
nemata become much more evident in most cases during 
