STUDIES ON CEYLON HAMATOZOA. 689 
kinetonucleus generally precedes the trophonucleus in divi- 
sion, but this is not by any means invariable. I cannot find 
any sign of the centrosomal function described by Franga 
for the Trypanosome in Hyla arborea.’ He describes a 
development of the Trypanosome resembling this early pro- 
cess in the leech, but in the case studied by him the kineto- 
nucleus appears to enter into what he considers definite 
centrosomal relations with the trophonucleus at the time of 
division. ‘This is quite absent in T. vittate. 
As already stated the flagellum develops with no very 
close regard to the exact stage of the nuclear divisions. It 
grows out rapidly, and in the stained preparations seems to 
develop by direct outgrowth from the kinetonucleus. The 
complicated figures met with at a certain stage in the deve- 
lopment of T. raiz in Pontobdella are here entirely 
absent. The products of the divisions show a certain 
amount of variation as regards the state of their development 
at the time of being set free. Pl. 16, fig. 11, is a very 
typical example of the more rounded forms, fig. 12 is 
another, and a third is shown in fig. 12a. 
Twenty-four hours after feeding the parasites are all 
flagellate forms varying considerably in shape, but most of 
them already showing an undulating membrane, and the 
kinetonucleus has migrated as a general rule pretty far 
back though it is still in front of the trophonucleus (PI. 16, 
figs. 11—15, 16, and 17). In the Glossiphonia this process 
is still quicker, and from this time on the infection in this 
leech begins to show the very wide range of forms so charac- 
teristic both of T. vittate and T. raie. 
A review of Pls. 16 and 17, figs. 13—26, will give some idea 
of the various types present. Long slender forms, some with 
exceedingly elongated bodies and the kinetonuclei showing 
considerable variation in their relation to each other, typical 
‘Trypanosomes often somewhat broad in shape, and small 
dumpy forms with short flagella, are all present together at 
1 (* Recherches sur les Trypanosomes des Amphibians,” ‘ Arch. de 
L’Inst. Roy. de Bact. Camera Pestana,’ T. i, Fasc. ii, 1907). 
