704, JOSEPH MANGAN. 
(Tiarella). In the former instance the chromatin shows an 
increase towards the approach of maturation. The nucleolus 
is single in the beginning in all cases, but in one type this 
body may subsequently fragment and undergo changes in 
staining properties, losing its affinity for basic dyes. Whether 
single or multiple the nucleolus vanishes before maturation of 
the egg. In very many cases chromatic bodies make their 
appearance in the cytoplasm close to the nuclear membrane, 
suggesting, particularly when the vesicle becomes achro- 
matic, that chromatin is cast out of the nucleus. Bearing 
the foregoing facts in mind, we may briefly review the 
phenomena in Millepora. 
A synapsis stage such as figured by Trinci (06) for 
Tiarella and Phialidium was not observed ; however, the 
subsequent appearance of branching and solitary chromatin 
strands in the expanding nucleus, found a close parallel in 
Millepora. 
In Millepora the chromatic strands gradually lose their 
staining capacity until the nucleus is practically, if indeed 
not absolutely, achromatic. About the time when the ovum 
has absorbed all its sister cells, the chromatin reappears as 
minute, diffuse granules. 
The single nucleolus vanishes at an early period in the 
growth of the oocyte. 
There is nothing suggestive of an expulsion of chromatin 
into the cytoplasm during the growth period, though of 
course an extrusion of non-chromatic substance could go on 
undetected. The achromatic phase of the vesicle is only 
temporary. 
B. Subsequent phenomena. 
Until the cytoplasm of the ovum is completely vacuolated 
its nucleus remains situated centrally, exhibiting deeply 
staining granules, uniformly throughout its substance, at the 
nodes of a fine achromatic reticulum (fig. 10). A nucleus 
which had begun to assume an oval shape, and was moving 
