724 ROBERT MCCARRISON. 
searching for them. The feces of 101 non-goitrous indi- 
viduals, living in the same locality, have also been examined. 
Amcoebee were present in twenty-nine of these; the infection 
was plentiful in eight, moderate in nine, and scanty in 
twelve. ‘I'he typical cysts hereafter described were also 
found in the only case of goitre from another locality which 
I have examined. 
Two distinct amcebe are found in the feces; these have 
not been distinguished in the live state: 
(1) A free amoeba which proceeds to encyst and develop 
into a typical 8-nucleated cyst. 
TEXT-FIG. 2. 
Trxt-Fie. 1.) 
ARE 
Text-fig. 1—Ameba I. Encysted ameba, showing single 
nucleus and port-wine staining area. 
Text-fig.2—Ameeba I. Encystedamcba. Shows the kidney 
shape of the port-wine staining area frequently observed. 
(2) A free amoeba which does not form obvious cysts, but 
multiples by division and budding. 
In addition, a third amoeboid body, enclosed in a charac- 
teristic capsule, is also present. Its affinities ave not clear, 
and I can only note its occurrence. 
' (Figs. 1-7 and 23 are drawn from freehand sketches of the living 
animals. The preparations were treated with iodine water. Drawings 
made under Leitz 51; in. oil-immersion, ocular No. 4. 
The remaining figures were drawn from fixed and stained preparations, 
under Zeiss 3 mm. apochromatic homog. oil-immersion, comp. oc. 12 
(x 2000). The drawings were made by Miss Rhodes, to whose skill in 
so accurately depicting the appearances observed I must pay a tribute. 
The preparations were stained with Haidenhain’s hematoxylin and 
Delafield’s heematoxylin. | 
