AMC@B# IN INTESTINES IN CASES OF GOITRE IN GILGIT. 725 
THe Am@pa IN THE FRESH SYratre. 
(a) The free amcebe.—I can only give a general account 
of the free amcebe in the live state, an account which covers 
both species. In specimens treated with iodine solution the 
organisms are more or less spherical. Their size varies 
between 124 and 20,4. Larger forms are occasionally seen. 
The protoplasm is granular, stains yellow with iodine, and is 
very rarely vacuolated. A differentiation of the protoplasm 
into ectoplasm and endoplasm can only be made out in those 
animals which show pseudopodia, and these are few. The 
TEXT-FIG. 3. 
TEXT-FIG. 4. 
ee - ; af 4 “. y 
Ps i 6 J te fy 
eal \ Reinet 
re Be. | 
S. 4. 
Text-fig. 3—Ameba I. Encystedameba. Bi-nucleated stage 
with large port-wine staining area. 
Text-fig, 4—Ameba I. Eneysted ameba. A stage not 
commonly met with, showing four nuclei and centrally placed 
port-wine staining area. 
protoplasm contains food-vacuoles and other inclusions, I 
have never seen any evidence of the ingestion of blood- 
corpuscles or of epithelium. The nucleus, where observed, 
is spherical or oval, and is sometimes surrounded by a narrow 
halo. In the larger organisms it measured 5 p-S yu. The 
characters of the nucleus are very distinct in the two species ; 
they have been studied only in stained specimens. 
(b) The encysted forms.—Live cysts of definite contour 
are very commonly seen; they represent stages in the life- 
history of Amoeba I, and show different appearances dependent 
on the length of time the animal has been encysted. These 
cysts are, as arule, perfectly spherical, but they may be oval. 
