AM@B# IN INTESTINES IN CASES OF GOITRE IN GILGIT. 727 
cyst. It usually lies in one hemisphere while the protoplasm 
is situated in the other, but it may be centrally placed. It 
appears sometimes to alter its position relative to the nuclei 
and to the cyst-wall. The port-wine reaction with iodine 
marks off the area from the rest of the protoplasm in a most 
distinctive way, for while the protoplasm is granular and 
stains yellow this area appears to be structureless. 
I have never been able to observe a division of the proto- 
plasm around the nuclei in the large 8-nucleated cysts. 
TEXT-FIG. 8. 
TEXxT-FIG. 7. 
| ona 
es TEXT-FIG. 9. 
9es 
fag A ee Ga . ah 
\ 4 Q ? 
| ] 
] : | . & 
ey a y 
i iS” > 
7. 8 = 9, 
Text-fig, 7—Ameba I. Encysted ameba. The nuclear 
division has resulted in the formation of five nuclei; compare 
text-fig. 17. 
Text-fig.8—AmebalI. Free ameba.  Sideview of organism 
showing finely granular appearance of protoplasm. The nucleus 
is seen surrounded by a narrow halo, and shows chromatin more 
or less evenly distributed with slight massing at four points of 
the periphery. A central karyosome is seen. 
Text-fig, 9—Ameba I. Unencysted ameba. Probably a 
stage in simple fission of free amceba. Chromatin massed at 
periphery of nuclei, which have beaded appearance. Karyo- 
some is seen; nature of included bodies not known. 
(c) The third organism referred to is less commonly found. 
The following are its main characteristics in both fresh and 
stained specimens. In the living state it is seen as a pear- 
shaped, oval, or spherical body, having a well-defined clear 
capsule. The protoplasm is granular and stains yellow with 
iodine. It is split up by a median fissure, on either side of 
