AMG@B& IN INTESTINES IN CASES OF GOITRE IN GILGIT. 729 
THe FIxeD AND STAINED AM@B2#. 
The material used for this part of the investigation was 
sent from Gilgit to England in Schandinn’s fixing reagent 
(saturated watery solution of corrosive sublimate two parts, 
alcohol one part). The methods of staining employed were 
Delatield’s and Haidenhain’s hematoxylin. The examination 
of the material was carried out in Professor Minchin’s 
laboratory at the Lister Institute, to whom I am indebted 
for help and advice. 
TExtT-FIG. 13. 
Text-Fia. 14. 
SS 
i y ie 
Text-fig, 13—Ameba I. Encysted ameba. Shows well- 
marked cyst-wall. A large hyaline mass occupying about one 
half of the cyst isseen. Chromatin masses lie above and below 
this area. Nuclei are seen lying at either side of cyst, each 
surrounded by halo. Note the reticular structure and granular 
appearance of nuclei. No karyosome is seen in either nucleus. 
Text-fig. 14—AmebalI. Encystedameba. One nucleus has 
proceeded to second division before the other. A common 
appearance of the nuclei —wheel-like—at this stage is well seen. 
Two chromatin masses in protoplasm. 
A study of the stained amcebe shows that there are two 
distinct species present. In view of the many opinions held 
with regard to intestinal amcebe I hesitate to describe these 
organisms under specific names. Nevertheless, I am inclined 
to think that Amceba J, which forms the 8-nucleated 
cysts, is the Entamceba coli, Schaudinn, and that Amceba 
II corresponds to the Entamceba histolytica, Schaudinn. 
Certain points in which they appear to differ from the two 
