AMGBA IN INTESTINES IN CASES OF GOITRE IN GILGIT. 731 
massed irregularly at the periphery of the nucleus. It is 
generally reticulate in character, and, as a rule, shows a 
distinct karyosome. I have not been able to satisfy myself 
as to whether division of the nucleus takes place in the 
unencysted organism or not. ‘T'ext-fig. 9 represents an orga- 
nism of a type only occasionally met with, in which it was 
impossible to make out the existence of a cyst-wall. This is 
probably a stage in simple fission of the free amoeba. Never- 
theless I hesitate to offer an opinion on the point, and simply 
draw attention to the figure. One point is certain, that in all 
Text-Fie. 17. 
be TrExtT-FIeé. 18. 
17. 
Text-fig. 17.—Ameba I. Encysted ameba; 8-nucleated cyst. 
The cyst-wall is thicker than in text-fig. 16. The nuclei are 
very clearly defined and each shows a karyosome. 
Text-fig. 18.—Ameba I. Eneysted amweba. Abnormal form 
showing twelve nuclei. 
organisms in which there were more than two nuclei a cyst- 
wall was always obvious. Unfortunately I have not noted a 
similar appearance in the living state, but, as I have said, my 
observations were then only diagnostic. 
The encysted amceba.—tThe earliest stages of encyst- 
ment which I have observed are shown in text-fig. 10. The 
chromatin is heaped up at the periphery of the nucleus, and 
here and there in the protoplasm dark masses presenting 
staining reactions similar to those of chromatin are occa- 
sionally found. The appearance of the protoplasm is dis- 
