fae ROBERT MCCARRISON. 
tinctive; 1t seems to be filled with spherical hyaline masses 
of variable size. It is difficult to offer an opinion as to the 
nature of these spherical masses. Such text-figuresas 11 and 
12 suggest that they gradually fuse, with the ultimate forma- 
tion of a large, clear hyaline body, as seen in text-fig. 13. This 
fusion appears to take place in those stages which represent 
the division of the nucleus after encystation. Jam convinced 
that the clear area (text-fig. 13) is that which gives rise to 
the characteristic port-wine reaction with iodine water in the 
living animal, ‘The hyaline body becomes less marked as 
TpxtT-FIG. 19. 
TEXT-FIG. 20. TExtT-FIG. 21. 
19. ii 2.0) 
Text-fig. 19 —Ameeba II. Two typical organisms. Shows pale 
staining nuclei surrounded by narrow halo. 
Text-fig. 20—Ameba II. Organism showing three nuclei and 
two darkly staining bodies. The precise nature of the latter 
objects is unknown; probably food-material. 
Text-fig. 21—Ameeba Il. A multi-nucleated organism. Nuclei 
of varying sizes. A stage of multiple division. 
the further development of the organism proceeds to the 
typical 8-nucleated form; ultimately it completely disappears. 
This hyaline body is at its highest development in the late 
bi-nucleated cyst. I regard this spherical mass as being of 
the nature of food material, a view which is upheld by 
Jurgens (2). A similar appearance has been described by 
Wenyon (8) in Entamoeba muris, and he has considered 
it to be “of the nature of food products which have not been 
thrown out of the animal.” It is questionable, however, 
whether the body seen by Wenyon is identical with that 
