748 R. ROW. 
no similar observations have been made for L. tropica, and 
the present memoir is the first account given of the cultural 
development of Wright’s bodies. As will be seen, the result 
is very similar to that obtained for the Leishman-Donovan 
body. It is of some interest, however, that the method of 
cultivation required appears to be quite different in the two 
cases, a fact which indicates that the transmission and mode 
of development are different in the two parasites. 
E. A. Mincnriy, 
Rovigno, March, 1909.] 
(1) Method of cultivation.—Material from the sore was 
planted in sterile sodium citrate solution (2 per cent.) and in 
blood-serum (human). Some of the cultures were left at the 
laboratory temperature (25°-28° C.), and some were incubated 
at 385° C. 
Those incubated at 35°C. and those planted in the sodium 
citrate solution did not show any growth; on the contrary, 
after twenty-four, forty-eight, and seventy-two hours they 
seemed to have disintegrated, so that not even a trace of the 
original parasites could be seen. In one case (a sodium 
citrate culture at room-temperature) large staphylococcus- 
like bodies were seen; they were probably contaminations, 
and were not investigated further. 
On the other hand, in the cultures in blood-serum at labo- 
ratory-temperature the parasites went through the develop- 
ment described in detail below; they increase in size, multiply 
greatly, and finally become Herpetomonas-like flagellates. 
(2) The parasites in the sore.—In smears from the 
juice of the sore stained with Giemsa’s stain the parasites are 
seen in all sorts of shapes—pear-shaped, oval, torpedo-shaped, 
and even spherical (figs. 1, 2, a—f, 3,a-g). They are found free 
outside the corpuscles and also in the large macrophages, in 
some of which they may occur in considerable numbers 
(fig. 1). The individual parasites consist of faintly staining 
protoplasm with macronucleus and micronucleus in various 
forms. The parasites appear to have a distinct capsule, 
