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ioe R.. ROW. 
and then become pear-shaped (fig. 7, b-h), with one end 
pointed. The nucleus divides and division of the body 
follows (fig. 7, 7-1). The pear-shaped parasite thus gives 
rise by fission to a slender form and a stout form (fig. 8, a). 
Hach of these divides again twice (fig. 8, b-i), so that from 
one parasite are derived eight flagellates—four long forms 
and four short and stunted forms (compare fig. 8,1). The 
stunted forms are short-lived, but the long forms persist in 
cultures of even 120 hours’ standing. All the division takes 
place before the flagellum is formed; after this event there 
seems to be no further multiplication. [This conclusion can 
only apply to the stunted cultures; in the more healthy 
cultures, as stated above, there is evidence of multiplication 
of the flagellated forms. Moreover, the vast masses of para- 
sites in the forty-eight-hour healthy cultures indicate that 
here many more than eight flagellates are derived from a 
single Wright’s body.] 
ADDENDUM. 
[When this memoir was completed and ready to send 
away I received, on March 27th, a third communication from 
Dr. Row, containing in concise form his conclusions, which I 
append here in his own words. Dr. Row exhibited his 
preparations and read a paper on them at the Medical 
Congress held in Bombay in February of this year.— 
Hi. A. M.] 
I conclude that the parasite of the oriental sore (Leish- 
mania tropica) and that of kala-azar (L. donovani), 
although apparently identical when examined in smears direct 
from the lesion, are distinct when examined in cultures, and 
for the following reasons : 
(1) The parasite of oriental sore, when fully developed 
into the flagellate forms under ordinary conditions of culture, 
is much longer and bigeer than that of kala-azar, where one 
meets with shorter and stouter forms, as a rule. 
(2) The parasite of oriental sore is more resistant to 
external conditions than that of kala-azar; in other words it 
