DEVELOPMENT OF PARASITE OF ORIENTAL SORE. 753 
is much less delicate, as it is possible to obtain develop- 
mental forms up to the flagellate stage from the parasite of 
oriental sore three days after its removal from the lesion, 
while the parasite of kala-azar, according to Rogers, dies 
within twenty-four hours after it leaves the spleen, if not 
cultured within that period. 
(3) The flagellum of the parasite of oriental sore is much 
longer, and presents more regular wavy undulations than that 
of kala-azar, where it is shorter with less regular undulations. 
(4) Although contamination of the material with extra- 
neous germs is inhibitory to the early developmental 
progress of the parasite of oriental sore, it is not so destruc- 
tive to its further development into flagellates as in the case 
of the parasite of kala-azar, where, according to Rogers, the 
slightest contamination with staphylococci is sufficient to 
destroy the culture. 
(5) The parasite of oriental sore develops into fully mature 
flagellate forms between forty-eight and seventy-two hours, 
while that of kala-azar takes twice as long if not longer. 
(6) The best culture medium (according to my results) for 
the parasite of oriental sore is haman blood-serum, by pre- 
ference that from tuberculous patients; while that for the 
parasite of kala-azar, according to Rogers, is sodium citrate, 
2-10 per cent., in sodium chloride solution 0°8 per cent., 
mixed with splenic blood. 
(7) The optimum temperature for the growth of the para- 
site of oriental sore is between 25° and 28°C., or even up to 
30° C., while for the parasite of kala-azar it is 22° C., or 
even less, according to Rogers. 
[Postscript (July 5th, 1909) —Since the above was sent 
to press I have been informed by Sir W. B. Leishman that 
the parasites of oriental sore have also been cultivated by 
Nicolle, whose memoir, however, I have not been able to see. 
—H.A.M.] 
