778 E. A. MINGHIN. 
first, and after him Prowazek and others, have attributed 
this to a similarity in origin and substance between the 
locomotor apparatus and the nucleus. According to Schaudinn 
(1904, p. 395) the entire locomotor apparatus (T'rypano- 
soma noctue) is a nuclear product, and the periplast stains 
red like the nucleus, because it contains myonemes origina- 
ting from the mantle-fibres of a nuclear spindle. Since 
Schaudinn wrote, the staining properties of the flagellum 
have generally been considered as due to its affinities with 
the chromatin of the nucleus. This is a good instance of the 
danger of founding conclusions of this sort on the results 
obtained by a single staining method; for when trypano- 
somes are stained with Twort’s combination of neutral red 
and licht-griin, the flagellum, blepharoplast and periplast 
stain green, in sharpest contrast to the two nuclei, which are 
both stained red (P]. 23); hence this method leads to con- 
clusions diametrically opposite to those that can be drawn 
from the results given by the Romanowsky combination. 
I cannot claim to have an expert knowledge of the chemical 
changes that go on, and the staining substances that result 
from them in the Romanowsky mixture; but certain con- 
clusions seem to me legitimate, and, indeed, quite obvious. 
In the first place the mixture contains eosin, or its equivalent, 
erythrosin. Kosin is not, however, a stain for chromatin, 
and it cannot be held accountable for the red coloration of 
the chromatin. When trypanosomes are stained with methy- 
lene blue and eosin by Chenzinsky’s method, the chromatin 
stains a pure blue colour. It is generally acknowledged that 
there is, in the Romanowsky combination, a red stain other 
than eosin at work which is accountable for the red stain of the 
chromatin. If it be true, however, that in the Romanowsky 
mixture at least two red stains are present, the one a stain 
for chromatin, the other not, it is evident that the fact of two 
structures being stained red by the mixture is no proof what- 
ever of any similarity in nature or substance between them. 
Hence the red stain of the flagellum and periplast is not 
proof in itself of their relationship to chromatin. 
