784, Kk. A. MINCHIN. 
after fixation with Schaudinn’s fluid and Mann’s fluid ; but 
as the preparations stained with it are so inferior to those 
obtained with iron-hematoxylin, it did not seem to me worth 
while to waste time on experimenting with it. 
Gentian violet and safranin.—I used these two stains 
made up as follows: 1 erm. of the stain in 90 c.c. aniline 
water with 10 c.c. absolute alcohol (Hermann’s formula). 
Films fixed in Hermann’s fluid or Schaudinn’s fluid, and 
stained in gentian violet for half an hour or so, were washed 
with abselute alcohol, then extracted in absolute alcohol 
saturated with orange G, then mounted in Canada-balsam 
alter passage through xylol. 
tesult (fig. 40): 'Trophonucleus a dark patch with karyo- 
some not distinct ; kinetonucleus indistinct ; flagellum faintly 
stained ; cytoplasm showing the very same appearance as 
alter Delafield’s haematoxylin. 
Other films, fixed as before, were stained in safranin at 
least twenty-four hours, washed with absolute alcohol, acidu- 
lated alcohol, and absolute alcohol again in rapid succession, 
then stained in gentian violet and differentiated with orange 
as already described. 
The only effect (figs. 41, 42) produced by the additional 
treatment with the safranin was to deepen the staining of the 
cytoplasmic granules, which assume a brownish, muddy tint, 
and so increase the marbled, blotchy appearance of the 
cytoplasm. 
Methylene blue and eosin.—Used according to Chen- 
zinsky’s method, as given in Bolles Lee’s ‘Vade-mecum?’ 
(5th edition, p. 222); films fixed with Hermann’s and 
Schandinn’s fluids. Griitbler’s methylene blue BX was used. 
Result : Exceedingly poor as show preparations, but inter- 
esting as showing the two nuclei and the cytoplasmic granules 
stained blue; no other parts of the body are stained at all, 
hence the trypanosomes appear shadowy and ghost-like, 
recognisable at first only by their nuclei and granules, but 
after careful examination the other parts of the body can be 
made out. The trophonucleus appears as a blue patch, with 
