794. 1. A. MINCHIN. 
These bodies are best seen after 'wort’s stain, which colours 
them red in the midst of the greenish cytoplasm (figs. 82-87). 
They are also well seen after iron-hematoxylin if the stain be 
but little extracted (figs. 28-30), and are most distinct when 
the trophonucleus appears as an evenly stained black patch 
without any detail; if, however, the extraction be carried 
further the stain comes out of them, and in preparations in 
which the karyosome stands out sharply from the tropho- 
nucleus the chromatoid grains are no longer visible (figs. 14, 
15, 25, 31, 32). With the Romanowsky stain the chromatoid 
vrains can seldom be made out; sometimes (figs. 61, 74, 80) 
afew red granules are to be made out, but more usually the 
cytoplasm stains an even bluish or purplish tint, which may 
mean either that the chromatoid grains are stained red, but 
are obscured by the dense blue colour of the general cyto- 
plasm, or that they also take the blue coloration of the 
Romanowsky stain. After Delafield’s hematoxylin the chro- 
matoid grains stain a dull violet like the nucleus; after 
gentian-violet-orange and safranin-gentian-orange they also 
stain like the. nucleus; after methylene-blue-eosin they take 
the blue colour. With all these stains, however, and also with 
iron-hematoxylin, the general cytoplasm is tinged in the 
same manner, probably owing to the presence of minute 
eranulations diffused through it which also take up the stain. 
Hence the chromatoid grains do not appear after the five 
staiuing methods mentioned as definite sharply marked 
bodies, but as blotchy, ill-defined patches which give the 
cytoplasm a marbled appearance. 
Twort’s stain alone, of all I have tried, differentiates the 
chromatoid grains clearly from the surrounding protoplasm, 
By this method they appear as coarse granules stained a faint 
reddish tinge, irregular in form and not sharply contoured. 
They vary in amount, being sometimes spread over the whole 
inter-nuclear region, and even extending into the pre-nuclear 
region ; in other cases there are only a few of them, occurring 
chiefly just behind the trophonucleus (figs. 82-87). These 
variations in the quantity of the chromatoid grains do not 
