796 E. A. MINCHIN. 
not stain, and is only visible by its refringence, and con- 
sequently disappears when mounted in refractive media. As 
to the nature or function of the granule, I am not able to offer 
any suggestions. 
In many trypanosomes in the standard preparations I find 
a distinct clear space or vacuole immediately in front of the 
kinetonucleus, varying in size, frequently absent, sometimes 
double (figs. 5-8). This is perhaps an artefact or post-mortem 
change, as I have not been able to see it in the living animal, 
nor does it occur in the ordinary stained preparation. 
(4) Locomotor Apparatus. 
Under this head are comprised the flagellum with its basal 
granule or blepharoplast, and the undulating membrane. I 
have already described above the general configuration of the 
flagellum and undulating membrane, and shall deal here only 
with points of minute structure. 
The blepharoplast is situated very close to the kinetonucleus, 
apparently touching it or even overlapping it; that is at least 
the position which it has in the standard preparations (figs. 1 
—8) and in all preparations which have been mounted without 
ever having been dried (compare figs. 62-69). On the other 
hand, in preparations which have been dried off, either before 
or after fixation, a considerable interval usually separates the 
kinetonucleus and blepharoplast (figs. 60, 61, 70, 73, 80, 81), 
a state of things which must be regarded as artificial. In 
view of the alleged purity of the technique of Salvin-Moore, 
Breinl, and Hindle (1908), I am surprised to notice such a 
condition very frequently in their figures. 
In preparations stained with iron-hematoxylin or other 
sharp nuclear stains the blepharoplast appears as a minute 
granule, a slight dilatation, often hardly visible, of the proxi- 
mal end of the flagellum (figs. 25-32, etc.). After Twort’s 
stain it is coloured green, like the flagellum itself, in sharp 
contrast with the nuclei, which are red (figs. 82-87). After 
the Romanowsky stain the blepharoplast is coloured red, like 
