THE STRUCTURE OF TRYPANOSOMA LEWISI. 805 
The following is a classification of the figures according to the method 
of fixation employed for the preparations : 
Osmic vapour simply: Pl. 21, figs. 1-8. 
Osmic vapour followed by absolute alcohol: Pl. 21, figs. 9-11; 
Pl. 22, figs. 60-69, 70, 71, 72, 74. 
Osmie vapour followed by sublimate-acetie (95:5): Pl. 21, 
figs. 14-18; Pl. 22, figs. 70, 73, 75. 
Osmie vapour followed by Schaudinn’s fluid: PI. 21, figs. 23, 
24, 45-53. 
Osmic vapour followed by Flemming’s fluid: PI. 21, fig. 39; 
Pl. 22, figs. 80, 81. 
Sublimate-acetic (99:1): Pl. 21, figs. 12, 13. 
Sublimate-acetic (95:5): Pl. 21, figs. 19-22; Pl. 23, figs. 82-84, 
88-91. 
Schaudinn’s fluid: Pl. 21, figs. 25-28; Pl. 22, fig.76; Pl. 23, fig. 85. 
Mann’s fluid without formol: Pl. 21, figs. 29-32; Pl. 23, fig. 86. 
Mann’s fluid with formol: PI. 21, figs. 33-35, 54-59; Pl. 22, figs. 
@(-79; Pl. 28, fig. 87. 
Flemming’s fluid: Pl. 21, figs. 36-38. 
Hermann ’s fluid: PI. 21, figs. 40-44. 
Figs. 19-22, 39, 60, 61, 70, 73, 74, 80, and 81 are from preparations 
dried off after fixation or after staining ; all others are from never-dried 
preparations. 
In studying the preparations, at least three trypanosomes were drawn 
from each, in order to take the average of the individual variations, 
which, though slight, must still be reckoned with, as seen from figs. 1-8 
and the table on p. 789. It was, however, neither possible nor desirable 
to reproduce all these figures. Where only one or two figures are given 
from a preparation it must be understood that they represent, as far as 
possible, the average proportions of the trypanosomes in the preparation. 
Hence such figures as 21, 22, 75, 79, ete., are not to be taken as repre- 
senting trypanosomes of small size, but as normal individuals showing 
the shrinkage produced by the method of preparation, and should be 
compared with others of the same date (see above) prepared in other ways. 
Av 2 1G 
Figs. 1-8.—“* Standard” preparations (see p. 759). 1 and 2 fixed 
with osmic vapour in a hanging drop; owing to the thickness of the 
preparation details could not be made out, but only the outline of the 
body and the flagellum could be drawn. 3 and 4, Fixed with osmic 
vapour in a hanging drop, then mounted on a slide and sealed up; the 
trophonucleus with its karyosome, kinetonucleus, and posterior refrin- 
gent granule are seen. 3 has a rod-shaped, 4 a rounded kinetonucleus. 
