806 BE. A. MINCHIN. 
5-8. Treated as 5 and 4, but with the addition of acidulated methyl- 
green solution to the preparation. The same details are seen as in the 
foregoing, with the addition of a vacuole or vacuoles immediately in 
front of the kinetonucleus, probably to be regarded as artefacts. 
With figs. 1-8 compare especially figs. 16, 35, 71, 72, 79, all drawn 
from preparations made from the same blood at the same time, but 
preserved in different ways. 
Fig. 9.—Outline of a specimen from the same preparation as figs. 62, 
63, showing the flagellum passing on the concave side of a body-curve 
at a point near its origin, and here having a sharp double bend. 
Figs. 10, 11.—Osmic vapour, absolute alcohol, iron-hematoxylin, 
never-dried. Note the distinct periplast-line. Compare especially with 
figs. 12, 15, 56, 57, 62-69, all preserved at the same time and from the 
same blood. 
Figs. 12, 13—Sublimate acetic (99:1); 12, stained Giemsa; 13, 
stained iron-hematoxylin; never-dried. Compare with figs. 10, 11, ete. 
Figs. 14, 15—Osmic vapour followed by sublimate-acetic (95 : 5), 
stained iron-hematoxylin; never-dried. Compare figs. 19-22, 39, 60, 
61, 70, 73, 80, 81, all preserved at the same time and from the same 
blood, , 
Fig. 16.—Fixation as in the last; stained Giemsa; never-dried. 
Drawn in outline to show the great diminution of size. Compare figs. 
1-8, etc.. made from the same blood at the same time. All the 
trypanosomes in the same preparation and in another treated in the 
same way at the same time show the same reduction in size. 
Figs. 17, 18.—Fixation as in 14-16; stained iron-hematoxylin ; never- 
dried. Compare with figs. 38 and 75, both preserved at the same 
time from the same blood. 
Figs. 19-22.—Fixed with sublimate-acetic (95:5) direct. 19, 20. 
Stained iron-hematoxylin; never-dried. 21, 22. Stained Giemsa, then 
dried off, showing extraordinary shrinkage. Compare figs. 14, 15, ete. 
Figs. 23, 24.—Osmie vapour, followed by Schaudinn’s fluid; stained 
iron-hematoxylin; never-dried. Note the very distinct periplast-line. 
Compare figs. 40-44. Preserved at the same time from the same 
blood. 
Figs. 25-28.—Fixed with Schaudinn’s fluid direct; stained iron- 
hematoxylin; never-dried. 25, much extracted; 26, 27, less; 28, still 
less extracted. Compare figs. 29-34, 76-78, preserved at the same 
time from the same blood. 
Figs. 29-32.—Fixed with Mann's picrocorrosive without formol; 
stained iron-hematoxylin; never-dried. 29, 30, less, 31, 32, more 
extracted. Compare figs. 25-28, etc. 
