THE STRUCTURE OF TRYPANOSOMA LEWISI. 807 
Figs. 53, 34.—Fixed with Mann’s picrocorrosive with formol; stained 
iron-hematoxylin ; moderately extracted ; never-dried. Two trypano- 
somes in the same field showing the relations of kinetonucleus, blepharo- 
plast, and flagellum very sharply. Compare figs. 25-28, ete. 
Fig. 35.—Treated as last. Rather more extracted. Compare figs. 
1-8, ete. 
Figs. 36-38.—Fixed in Flemming’s fluid direct; stained iron- 
hematoxylin; never dried. 56, 57, compare with figs. 10, 11, ete. 58, 
compare with figs. 17, 18, etc. 
Fig. 39.—Fixed with osmic vapour followed by Flemming’s fluid ; 
stained iron-hematoxylin; never-dried. Compare with figs. 14, 15, ete. 
Figs. 40-44.—Fixed in Hermann’s fluid direct; 40, stained gentian 
violet and orange; 41, 42, stained safranin, gentian violet, orange; 45, 
44, stained iron-hematoxylin; never-dried. Compare figs. 23, 24, ete. 
Figs. 45-55.—Nuclei of trypanosomes from the same preparation as 
figs. 23, 24. 45 shows the most usual condition, occurring in about 90 
per cent. of the trypanosomes. 
Figs. 54-59.—Nuclei of trypanosomes from a companion preparation 
to that from which figs. 35, 54 were drawn; treated in the same manner, 
but the stain rather more extracted. 54 represents the most usual con- 
dition. 
PLATE 22. 
All figures drawn from preparations stained with the Romanowsky 
stain, either Giemsa’s method (G.) or azure-erythrosin (A. E.). 
Figs. 60, 61.—Fixed with osmic vapour, then dried off; fixed with 
absolute (G.). Compare with figs. 14, 15, ete. 
Figs. 62, 63.—Osmie vapour; absolute alcohol; never-dried (G.). 
Compare with figs. 10, 11, ete. 
Figs. 64-69.—From a companion slide to the foregoing; more 
extracted with acetone (see pp. 775-777). 
Figs. 70-72.—Trypanosomes showing folds of the deeply-stained 
periplast simulating fibrils (see p. 797). 70, fixed osmic vapour, then 
dried off; sublimate acetic (95:5), stained (G.); from a companion 
preparation to that from which fig. 73 is drawn. 71, 72, osmic vapour, 
absolute alcohol (G.); never-dried. Compare figs. 1-8, etc. 
Fig. 73.—Osmic vapour, sublimate-acetic (95 : 5), stained (G.); dried 
off after the osmic vapour. Compare figs. 14, 15, etc. 
Fig. 74.—Trypanosome with three trophonuclei; osmic; absolute 
aleohol (G.) ; dried off. 
