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Bio-Chemistry. — ''Experiments on Anaphylaxis with Azoprotéins'\ 

 By K. Landsteiner. (Communicated by Prof. C. H. H. Spkonck.) 



(Communicated at the meeting of January 28, 1922). 



In previous articles the writer described methods for producing 

 immune sera, acting upon known chemical groups. These methods 

 are based upon the use of antigens, consisting of proteins, which 

 are chemically combined with substances of simple constitution. ') 



As already indicated, the question suggests itself as to whether 

 anaphylaxis can be produced by these compounds and what is 

 the action in anaphylaxis (sensitization and shock) and antiana- 

 phylaxis of each of the two components of the antigen, viz. the 

 proteins and the simple substances combined with it. The significance 

 of these problems for the theories of immunity and anaphylaxis and 

 the knowledge of the condition of hypersensibility produced by 

 simple substances is evident (cf. Doerr) '). 



The experiments presented here') form part of a series, the car- 

 rying out of which has been delayed because of external circum- 

 stances. 



The guinea pigs were sensitized by means of azoprotein *) prepared 

 from horse serum and p-arsanilic acid (1 gr. of atoxyl for 100 cc. 

 of serum). 



For the second injection an azoprotein formed by combining fowl 

 serum and p-arsanilic acid was employed. The use of a number of 

 other azoproteins was rendered diflicult because of their toxicity 

 when injected intravenously. 



Results: It was found to be more difficult to produce the ana- 

 phylactic state with the substances mentioned above than with the 

 proteins usually employed, and in the experiments to be described 

 it was necessary to make three intraperitoneal injections, correspond- 

 ing to 0.5 to 1.0 cc. of serum each, in order to produce consider- 

 able effects. 



») Zeitschr. f. Immun. 26, p. 258 (1917), Biochera. Zeltschr. 86, p. 343 (1918). 



2) Doerr, Schweiz, med. Wochenschr. 1921. No. 41. 



') Details will be given later. 



*) 1. c. Bioch. Zeitschr. 86, p. 359. 



