Bacteriology. — ''Studies on the hactfriop/ufi/u.s' of d'Herei,i-k." II. 

 By J. W. Janzkn and \j. K. Wolff. (Coiumuiiioated by 

 Prof. C. Eykman). 



(Communicated at the meeting of March 25, 1922). 

 II. The BdcteriopJuiyus toit/i regard to jiagellates. 



We have heei» informed by d'Hkkklle that the water of' some 

 Indian rivers possess the bacteriophagus propeities. 



lu connection with that we have considered it of importance to 

 see how far flagellates ont of a mixlnre of bacteria and bacterio- 

 phagns also eat the latter. 



Ip. order to do this we prepared a snspension of dead typhoid 

 bacilli in saltsolution, and to a third pait of this we added 2 cM' 

 canalwater; a second portion was mixed with liacteriophagiis and 

 2 cM" canalwater; a third |)Ortion was only mixed with the same 

 quantity bacleriophagns as the second. 



After 9 days the two first portions had become considerably 

 clearer and we conid distinctly show flagellates in the microscopic 

 preparation. 



Now dilutions were made, the number of bacteriophagus germs 

 of which was stated in the wellknown way. 



We found : 



II emulsion -|- canalwater -\- bacteriophagus in 



J/400.000 cM" 71 islands. 



III emulsion -)- bacteriophagus in 1/400.000 cM' 380 islands. 

 With another trial we found after 14 days: 



II emulsion -|- canalwater -|- bacteriophagus in 



1/4000 mill. cM" 120 islands. 



III emulsion -[- bacteriophagus in 1/400 mill. cM' 50 islands. 

 This numbers are of the same i-ange ; the differences range within 



the mistakes of the experinjents. 



The suspensions without canalwater remained absolutely turbid, 

 because the bacteriophagus does not affect dead bacilli. 



From these two experiments we wish to conclude that the bacterio- 

 phagus is not being affected by flagellates. 



