173 



II. 1st culture of bacteriophagus germs 

 2nd „ „ „ 



OlQ n If n 



18 milliard per cM3 

 02 „ „ „ 

 disappeared 



Finally an experiment vvitli old non resistant bacilli. 



A. 14 days old bacilli Sm in broth. 



B. 6 hours old bacilli Sm in broth. 



Our typhoid bacilli that are resistant by nature to the bacterio- 

 phagus do not even lose their resistance after being subcultured 

 repeatedly in contradistinction to what d'Herellk tells us in his 

 book (pag. 67) about the bacilli, who are been made resistant by 

 influence of the bacteriophagus. 



V. About big and small islands. 



0. Bail and T. Watanabe have said that, in plating a mixture 

 of bacteriophagus and bacteriacultures on agarplates, the islands are 

 not always equally big, but that sometimes big ones, medium ones 

 and small ones are to be found. 



They have tried to cultivate the bacteriophagus of these islands 

 purely; they say tliat they have succeeded in doing this with the 

 small islands, not with the big ones however. 



We too had already been struck by this before Bail's communi- 

 cation reached us, and we have tried to isolate these bacteriophagi, 

 forming big and small islands, from each other, but we did not 

 succeed. We have stated though, that it could not be possible in 

 our cases, as a bacteriophagus which exclusively formed big islands 

 with regard to one typhoidstrain, made nothing but small islands 

 with regard to another typhoidstrain, and as to a third, both big 

 and small ones. So we do not believe that Bail's and Watanabe's 

 explanation is right, but we think the difference in size of the 



