194 



subsequently \'\eiglied. This inaleiial was used for making counting- 

 tests by sowing the inicro-organisins on nutrient gelatin. The counting 

 of" the microbe-colonies for the aerobic plate-cullures took j)lace after 

 48 aud 72 hours, after which there was hardi}- any increase of the 

 colonies worth mentioning. 



The anaerobic culture plates for the conn ting- tests were made 

 after Wright aud Buhki's ') culture method, modified by me. As 

 this strictly anaerobic method of cultivation yields very good results, 

 it will not be amiss to state our procedure. 



In a glass box closed tightly by a glass stopper with a ground 

 rim a smaller petri-dish is j)laced containing a, solidilied culture- 

 medium on which the anaerobes are sowii in streaks. The circular 

 open space left round the dish is first stopped up with non-absorbent 

 cotton- wool on which a layer of absorbent cotton- wool is laid. The 

 latter is soaked with 20 "/o potassium hydrate and finally with an 

 equal volume of 20 '/o pyi'Ogallic acid. 



Throughout this procedure the petri-dish remains covered. After 

 the cotton-wool has been soaked with pyrogallic acid the dishcover 

 is removed, while the glassbox is closed by its cover-glass of 

 which the glass-rim is smeared with vaselin. The rim of the glass- 

 box may also be shut off with paraffin after the lid has been 

 adjusted. In order to facilitate the opening of the glass-boxes, the 

 wall is provided with a little hole which is shut off with paraffin 

 and is opened again before taking off the lid of the box, in 



1) J. H. Wright. A method for cultivation of anaerobic bacteria. Centralbl. f. 

 Bakt. Ite Abt. 29, 1901, pg. 61. R. Burri. 2te Abt. 1902. 8, pg. 533. 



