278 



udders are connected with the transit of bioodplasma-components 

 in abnormal milk. Wiiile 100 c.c. normal milk — after removal 

 of casein and fat with the aid of potassium-alum contain according 

 to our investigations about 14 — 20 mgrs. of tryptophane, as much 

 as 348 mgrs. occurred in the milk-samples after the injection of 

 silvernitrate, that is about twenty limes more. 



The detei'inination of tlie trjptopiiane-content, easily executed by 

 the colorimetrical method of von Fürth and Nobet/), is no doubt 

 one of the most accurate methods for examining the normality 

 of milk. 



The foregoing experiments tend to show that the anomalies 

 characteristic of streptococci-containing milk, arise also fron) sterile 

 inflammation of the udder-tissne, so that streptococci need not always 

 be essential to the occurrence of similar anomalies. The question 

 whether, in the case of streptococci-mastitis, these bacteria are very 

 often only of secondary importance can of course not be answered 

 on tlie basis of this investigation. 



From the CJiemical Laboratory of the Veterinaj^y 

 University of Utrecht. 



1) Biochem. Zts. 109. 103. (1920). 



