41 
April 16 and 23¢ 1917. A repetition of the experiment of 
April 10%. The results of these three experiments are represented 
Figure 12, 
minutes. 
in fig. 12. As will be seen clearly in the curves of April 10% and 
27th, there is first a decline, after which the reaction proceeds nor- 
mally again. As discussed above the inference of this is, that during 
some time interval the decaying enzyme particles, which cause the 
synthesis, may prevail, and that after their total decay (or perhaps 
restoration) the normal enzyme action sets in again. 
In order to test this conception more directly the following experi- 
ment was carried out. : 
In one row of tubes the rate of hydrolysis of an 0.01 °/, urea 
solution in 8°/, phosphate of p= 8.13 was investigated as before. 
Another row of tubes, each also with 10 c.c. of the same Soja- 
extract-phosphate-solution had been placed with all the other tubes 
at the same time in the same bath of 27°. Immediately after taking 
out a tube of the first row for analysis, 2 c.c. urea solution of 
0.06°/, were introduced into a tube of the second row and the 
reaction in this one allowed to proceed for 120 minutes. In this 
way it was possible to get some information about the rate of 
decay of the enzyme. 
From Fig. 13 it will be seen, that the nearly horizontal part of 
