128 
Correcting the figures of March 14th by this value we get: 
ammonia converted into urea by 
3 g. of Soja 1,33 ee. + N 
6 g. of Soja 2.02 ee. a5 N. 
March 28th 1916. In the large cylinders 11.572 g. of ammonium 
carbonate and 100 c.c. of water. In the small cylinders 190 g. of 
H,SO, of about 0,8 N (1 gram = 7,85 c.c. +; N). 
In two of the six large cylinders 6 g. of Soja-meal, in two others 
12 grams. 
After 24 hours’ standing at room temperature 250 c.c. of saturated 
potassium carbonate and a few drops of octyl-aleohol were introduced 
into each and the ammonia blown over for 48 hours. 
Results: 
TABLE 23 
c.c. NH3 75 N in: 
wanes 1______| Corrected | 
_ First (Second, _ NH3 converted 
cylinder |cylinder | Mean nk | into urea 
ee ee | 
| | 
Amm. carb. alone | 1462.4 | 1462.5 | 1462.45 | 
Amm. carb. + 6 g.ofSoja| 1460.7 | 1460.5 | 1460.6 | 1459.6 | 2.85 
Amm. carb. + 12g.of Soja, 1458.5 1458.4 | 1458.45) = 1456.5 | 5.95 
May 16% 1916. A solution of about 95 g. of ammonium carbonate 
in 1 Litre of water was prepared and saturated with carbonic acid. 
Two large cylinders, each with 100 e.c. of this solution and 250 c.c. 
of saturated potassium carbonate were connected with small cylinders 
containing each 174 g. H,SO, 0,8 N and all the ammonia blown 
over into the acid by passing an air current for 48 hours. 
Four large cylinders (A) received each 100 c.c. of the same ammo- 
nium carbonate solution and 6 g. of Soja-meal. 
Four others (B) each 100 c¢.c. of ammonium carbonate solution 
and 12 g. of Soja-meal. 
After 2 days at room temperature one cylinder A and one cylinder 
B were analysed by introducing 250 c.c. of saturated potassium 
carbonate and blowing over the ammonia into 174 g. acid for two 
days. In the same way one A and one B were treated after 3 days, 
and two cylinders A and two cylinders 5 after 4 days. See Table 24. 
Evidently the equilibrium is scarcely reached in about 3 days. If 
the activity had remained unchanged, it might have been reached 
much sooner, or probably no synthesis at all had been effected. 
According to what we have seen above, the urease had been gradually 
