458 
sodium chloride, after passing the different alcohols, they were 
enclosed in paraffin and then they were cut into series of 10u and 
the sections were stained according to HerDeNHAIN’s ironhaematoxylin 
method. I counted the number of nuclei of some of these sections 
over a length of 1 mm. from the roottop and I fixed the number 
of mitoses. | took care to count only in the central sections. Table 
I and II give my results. 
TABLE I (in daylight). 
Total Total | Spirema | T 
Time. | number of | number of ‚and loose Monaster.' Diaster. | ne 
nuclei. mitoses. chrom. Meh 
| ru | 
| | 
Seam. | 4000 — — — — — 
lam. | 434 139 53 53 17] deels 
3 p.m 2290 41 26 14 5 2 
TABLE II (in the dark). 
Total Total = Spirema | TWo 
Time. number of | number of ‘and loose} Monaster.| Diaster. Paine 
nuclei. mitoses. chrom. : 
6 a.m. 4702 210 125 66 19 0 
12 a.m. 4204 180 147 21 20 4 
6.30 p.m. | 4043 124 65 29 10 20 
In order to compare these figures, I expressed them in percentages 
in the following tables. Karsten takes the average of his countings. 
In my opinion it is more exact to express these facts in percentages, 
just as Mrs. Droogiever FoRTUYN-VAN LeypEn does, for it is most 
improbable that Karsten always examined the same number of cells. 
TABLE Ill (in daylight). 
Total’ =|... Total Spirema ee aR 
Time. number of | number of |and loose|Monaster.| Diaster. | Vite; 
nuclei. mitoses. chrom. | 1 
ne ee 
8 a.m 4000 0.00 % | 0.00 % | 0.00 J | 0.00 % | 0.00 % 
il a.m 4345 AOT sed LD lng 1e AFG TIDE ig 0x97 5 
3 pm 2290 2505 fy LS 0.61 , 0.21%, 0.08 , 
