82 



the culture being grown on a phosphate-containing medium. The 

 amounts are such as to render the turbidity in the two flasks apparently 

 equal. Temperature 20° — 25°. The carbonic acid output is read 

 above the level of the mercury. After the lapse of five hours not 

 yet an appreciable fall of the mercury-column ; after 48 hours with 

 A a fall of 4 c.c, with B 4^ c.c. (to the level of the mercury- 

 reservoir) ^). At the termination of the experiment in .4 exclusively 

 yeast-cells without volutin ; in i^ a large part with volutin. 



26 January 1917. In flask A 25 c.c. 0,5 perc. glycose, 0,05 perc. 

 MgSO^, 0,5 perc. aspara'gin and a trace of KNO^, in flask B the 

 same liquid -|- 0,1 perc. KH^PO^. In A inoculation with Torula 

 monosa without volutin from a phosphate-free asparagin medium. 

 In B Torula monosa that has been incubated from 29 June 1916 

 to 23 Jan. 1917 with a phosphate-free medium and by transplanting 

 into malt-agar has again grown rich in volutin. Carbonic-acid pro- 

 duction read above the level of the mercury. Temperature 20°— 25°. 

 Fall of the mercury-column with ^ in 6 hours 2^ c.c. with ^ 3 c.c. 



3 April 1917. A flask of 25 c.c. 2,5 perc. glycose, 0,2 perc. 

 peptone, 0,05 perc. MgSO, and a trace of KNO,. Inoculation with 

 volutin-free culture of Torula monosa, which had been transplanted 

 on March 30 on a phosphate-free medium and which originates from 

 a phosphale-free culture dating from June 19, 1916. After two hours 

 fall of the mercury-column = 3^ c.c. (which means fermentation of 

 a volutin-free culture of rather more than nine months). 



13 March 1917. In flask A and B each 25 c.c. 2,5 perc. glycose, 

 0,2 perc. peptone, 0,05 perc. MgSO^ and a trace of KNO3. yl. inocu- 

 lated with a second inoculation from a volutin-free culture, B. from 

 a volutin-rich culture. Apparently equal turbidity in the two flasks 

 at the commencement of the experiment. After one hour both mer- 

 cury-columns are fallen 1 c.c, after 18 hours ^ =^ 3 c.c. 5 = 4^ c.c 

 (down to the mercury level in the reservoir.^) 



March 1917. Beer-yeast from the brewery, kept under 10 perc. 

 cane-sugar, in which initially a marked fermentation has taken place 

 (microscopically coarse volutin-granules; no appreciable contamination 



1) The determinations were read from non-graduated tubes, on which the level 

 of the mercury was marked with anilin pencil. The fall was expressed in centi- 

 metres ; 4 cm. corresponded with a fall of 4 c.c. of mercury. Minute quantitative 

 determinations cannot he made in this way, which indeed was not aimed at. hi 

 the case of a fall down to the level of the mercury-reservoir, the amount of car- 

 bonic acid given off may, of course, have been larger than we were able to read. 

 Therefore, for a more accurate quantitative comparison some determinations were 

 made after Benedict (vide p. 83). 



